2号、3号和4号外显子被替换成一个puro cassette。通过在脾细胞和胸腺细胞中进行 Western blot 分析,确认了蛋白表达的缺失。(来源:J:169170)
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Exons 2, 3, and 4 were replaced with a puro cassette. The absence of protein expression was confirmed by western blot analysis on splenocytes and thymocytes. (J:169170)