Tg(Slc2a4-cre)546Dac

A 2.4 kb construct containing the Slc2a4 (GLUT4) promoter driving expression of cre recombinase was injected into C57BL/6 x FVB fertilized eggs. Two founders were produced, 535 and 546, and characterized for transmission and recombination. Line 546 showed autosomal transmission and line 535 showed X-linked heritability. (Both lines underwent germ line recombination when transmitted through the dam. When transmitted through the sire, both transgenes were liable to stochastic em- bryonic activation, leading to generation of animals with varying degrees of chimerism.) Line 546 recapitulated endogenous Slc2a4 expression, exhibiting recombination in skeletal muscle, epididymal and subcutaneous white adipose tissue, brown adipose tissue, heart, and kidney. However, recombinase activity was observed ectopically in the ovarian corpora lutea, myometrium, nuclear layers of the retina, acinar cells in the exocrine pancreas, and salivary gland. Line 546 was studied in greater detail. (J:169404)