A loxP site was inserted upstream of exon 15. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 15. Flp-mediated recombination removed the neo cassette leaving exon 15 floxed. (J:169296)
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A loxP site was inserted upstream of exon 15. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 15. Flp-mediated recombination removed the neo cassette leaving exon 15 floxed. (J:169296)