将一个新的neo cassette插入了exon 6,这个exon编码SNAREmotif的一部分。蛋白质表达的缺失通过在E18.5脑组织提取物中进行 Western blot 分析得到了确认(来源:J:169107)
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A neo cassette was inserted into exon 6, which encodes part of the SNARE motif. The absence of protein expression was confirmed by western blot analysis on E18.5 brain extracts. (J:169107)