Mbd4/Ift122^tm1Abc

A genomic fragment encompassing exons 1, 2, and 3 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the entire 5-methylcytosine binding domain and a portion of the catalytic domain. RT-PCR confirmed the absence of expression of both genes in homozygous MEFs. (J:86981, J:143544)