将lacZ基因座的靶向载体通过同源重组插入到了第15外显子。 Western blot结果显示,纯合小鼠大脑中没有检测到蛋白质表达。(来源:J:167904)
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A targeting vector, containing a lacZ gene cassette, was inserted into exon 15 via homologous recombination. Western blot analysis confirmed the absence of protein expression in brains from homozygous mice. (J:167904)