Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj

The TCF/Lef:H2B-GFP transgene was designed with six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) DNA binding site and a heat shock protein 1B (Hspa1b) minimal promoter driving expression of an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene), followed by a simian virus 40 (SV40) polyadenylation signal. (J:167970)