Ostf1^{tm1(NCOM)Cmhd}

The insertion of NorCOMM cassette pNTARU created a deletion of size 3275bp between positions 18570945-18574220 of Chromosome 19 (Build GRCm39). This deletion results in the removal of functionally critical exons 3 and 4. Western blots confirm the absence of protein expression from this allele (other than the beta-galactosidase reporter). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)