通过Flp介导的重组移除了neo cassette,而Cre介导的重组则去除了exon 2。蛋白表达的缺失通过 Western blot 分析和免疫组化进行了确认(来源:J:167767)
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Flp-mediated recombination removed the neo cassette, and cre-mediated recombination removes exon 2. The absence of protein expression was confirmed by western blot analysis and immunohistochemistry. (J:167767)