Exon 1被替换成lacZ和FRT引导的neo cassette,随后通过Flp介导的重组移除了neo cassette。蛋白表达缺失通过在E15.5和P14脑组织的 Western blot分析得到了确认(来源:J:166980)
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Exon 1 was replaced with a lacZ and FRT-flanked neo cassette. Flp-mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on E15.5 and P14 brain extracts. (J:166980)