含有Slc32a1(维亚塔)基因座的BAC RP23-392P11通过同源重组插入了Cre重组酶序列、终止密码子和Actin-related protein 5同源基因(Actr5)的polyadenylation序列,位于起始密码子下游。经线性化后,BAC中仅包含维亚塔基因和Actr5基因的剪切片段。这种经过修改的109kb BAC被注入了FVB/N雌性卵母细胞。转基因动物的Slc32a1和Actr5表达水平与野生型小鼠相似。已鉴定出2.1号克隆并进行了扩增(来源:J:166851)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
FVB/N
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28

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
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Year
IF
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