Tg(ACTB-tTA2,-MAPT/lacZ)1Luo

The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene was designed with the human cytomegalovirus (CMV) enhancer/chicken beta-actin core promoter (pCA), a loxP-flanked neomycin resistance gene (neor) with transcriptional STOP cassette, a modified tetracycline-regulated transactivator gene (tTA2), an internal ribosome entry site (IRES), and a bovine microtubule-associated protein tau with beta-galactosidase fusion protein (tau-lacZ). The transgene was further modified to have two copies of a ~250 bp chicken beta-globin HS4 insulator sequence (separated by an ApaLI restriction site) on each side of the transgene. The 5' insulator pair is flanked by Saw1 and Kpn1 restriction sites, while the 3' insulator pair is flanked by Spe1 and Asc1 restriction sites. (J:167275)