在exon 2上游插入的loxP位点丢失了,导致下游的exon 3后有一个含floxed neo cassette的片段。蛋白质表达缺失通过 Western blot 分析得到了确认(来源:J:167023)
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The loxP site inserted upstream of exon 2 was lost leaving a floxed neo cassette downstream of exon 3. The absence of protein expression was confirmed by western blot analysis. (J:167023)