在exon 2的上游插入了一个带有5' loxP位点的FRT引导的neo cassette,然后在exon 3的下游插入了另一个loxP位点。通过Flp介导的重组,neo cassette被移除,剩下exons 2和3被标记为可剪接。(来源:J:166367)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count