One loxP site was inserted just 5' of exon 9 and an FRT flanked neomycin selection cassette was placed in intron 10 followed by a second loxP site. The selection cassette was subsequently excised from properly targeted ES cells usin flp recombinase, leaving exons 9 and 10 flanked by loxP sites. Mice were then crossed to transgenic mice expressing Tg(CAG-cre)13Miya to delete exons 9 and 10. (J:165706)