Tg(Alb1-GP33/H2-K^b)1Hpi

The mouse albumin promoter fused to the promoterless genomic H-2Kb gene, in which the signal sequence of the Kb molecule encoded by exon 1 was replaced by the leader sequence (aa 1-60) of the LCMV glycoprotein (GP) encoding the GP33 epitope (aa 33-41). The construct does not encode a functional Kb molecule, since insertion of the LCMV GP fragment leads to a frame shift and a stop codon in the second exon. RT-PCR analysis revealed GP33 transgene expression in the thymus and liver (100-1000x the level of the thymus). (J:162605)