通过将Cyp51小鼠与Tg(EIIa-cre)C5379Lmgd小鼠杂交,剪除了exons 3和4。基因表达的缺失通过定量RT-PCR得到了确认。 Western blot结果显示没有能免疫检测到的蛋白质。(来源:J:173366)
查看原文 参与反馈
Exons 3 and 4 were excised by crossing Cyp51 mice to Tg(EIIa-cre)C5379Lmgd mice. Absence of gene expression was confirmed by quantitative RT-PCR. No immunologically detectable protein was observed on Western blots. (J:173366)