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A point mutation of a T to an A at 919, that results in a Tyr to Asn amino acid change at residue 307 and a floxed neomycin cassette within intron 2 were inserted by homologous recombination. Quantitative real time PCR showed that mice expressed only 40% of the wild-type level of mRNA. Western blotting showed a reduction in the band intensity in brain, skeletal muscle and heart relative to wild-type. (J:164634)