Hprt1^{tm37(lacZ)Ems}

The "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. (J:144244, J:164356)