在AflII-KpnI限制片段的theta区域,用一个由loxP序列标记的诺卡因抗性基因座取代了前体。通过Cre介导的重组,去除了诺卡因基因座。半定量的RT-PCR和RNA原位杂交在E8.5时尾状前脑原基中证实了转录表达的缺失,而在E9.5和E10.5时,表达水平有所降低。(来源:J:163926)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count