Anpep^tm1.1Lsha

A loxP site was inserted upstream of exon 4 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 13. Cre mediated recombination removed exon 4 through exon 13 as well as the neo cassette. The absence of protein expression was confirmed by western blot analysis on kidney, spleen, liver, and intestine extracts. (J:163945)