目标基因的第二个外显子被1号内含子的LoxP位点和一个FRT-介导的PGK-Neo序列,随后是另一个LoxP位点的2号内含子 flank by 这些元件。通过Flp介导的重组,选择性载体被移除,使得外显子2得以敲除。(来源:J:163812)
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Exon 2 of the targeted gene was flanked by a loxP site in intron 1 and a FRT-flanked PGK-Neo sequence followed by a loxP site in intron 2. Flp mediated recombination removed the selection cassette leaving exon 2 floxed. (J:163812)