从13到16的外显子被替换成一个纯化载体,并连接了一个3'的Hprt小基因。通过脑组织提取物的 Western blot 来确认没有蛋白质表达。(来源:J:163721)
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Exons 13 through 16 were replaced with a puro cassette and 3' Hprt minigene. The absence of protein expression was confirmed by western blot on brain extracts. (J:163721)