从E12.5期鼠胚胎成纤维细胞的提取物中,通过 Western blot 实验确认了蛋白质表达的缺失,具体是通过替换2到5号外显子形成了一个neo cassette(来源:J:163302)
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Exons 2 through 5 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot on extracts from E12.5-derived mouse embryonic fibroblasts. (J:163302)