A FRT flanked neo cassette with a 5' loxP site was inserted within intron 1 and an additional loxP site was inserted within intron 2 by homologous recombination. The selection cassette was removed by flpe mediated recombination. (J:163063)
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A FRT flanked neo cassette with a 5' loxP site was inserted within intron 1 and an additional loxP site was inserted within intron 2 by homologous recombination. The selection cassette was removed by flpe mediated recombination. (J:163063)