同源重组复制了基因。Cre介导的重组移除了带有floxed neo cassette的片段,留下基因的部分区域被标记。 Western blot结果显示蛋白表达有所增加。(来源:J:119643)
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Homologous recombination duplicated the gene. Cre mediated recombination removed the floxed neo cassette leaving a portion of the gene floxed. Western blot analysis confirmed increased protein expression. (J:119643)