Pms2^tm1.1Wed

An E702K mutation was introduced into exon 12 disrupting the DQHA(X)2E(X)4E metal binding motif. In addition, a floxed hygro/neo selection cassette was introduced in intron 12. Cre mediated recombination then removed the selection cassette. RT-PCR and qPCR indicted that expression levels of the mutant allele were similar to wild-type in MEFs and primary B cells. Western blot analysis indicated about a 25% reduction in protein levels in the spleens, brains, and testes of homozygous mice compared to wild-type tissues. (J:162378)