这个等位基因是通过将Ppibtm1Rjb与携带MMTV-Cre转基因的老鼠杂交产生的,通过靶向的loxP位点删除了第三个外显子。在胸腺细胞中通过 Western blot 实验证实了基因产物的完全丢失,这是一项无功能的等位基因。(来源:J:161748)
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This allele was created by crossing Ppibtm1Rjb with mice bearing MMTV-Cre transgene to remove the third exon franked by targeted loxP sites. Complete loss of gene product was confirmed by western blot in thymocytes. This is a null allele. (J:161748)