通过Cre介导的重组,去除了neo cassette以及序列2到7的外显子。蛋白表达的缺失通过在小鼠胚胎成纤维细胞提取物中进行 Western blot 分析得到了确认。(来源:J:155178)
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Cre mediated recombination removed the neo cassette and exons 2 through 7. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblast extracts. (J:155178)