Tg(tetO-tdTomato,-Syp/EGFP*)1.2Luo

The TRE-Bi-SG-T transgene was designed with a tdTomato protein sequence on one side, and a Syp-mut4EGFP fusion protein sequence on the other side of a bi-directional tetracycline-responsive promoter (tetO; also called tetracycline operator, tet-operator, or tetracycline-responsive element [TRE]) with CMV minimal enhancer-less promoter. The tdTomato is tagged with three Myc epitopes at its C-terminus and followed by a polyadenylation signal (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). The Syp-mut4EGFP fusion protein is a full-length mouse synaptophysin (Syp) fused to a mutant enhanced green fluorescent protein (mut4EGFP; harboring the V163A/S175G codon changes for increased ability to fold properly at 37 degrees C) and followed by a polyadenylation signal. The TRE-Bi-SG-T transgene was further modified to have two copies of a 1.2 kb chicken beta-globin HS4 insulator sequence (separated by a PacI/AscI/PmeI polylinker) on each side of the transgene. (J:161840)