这个序列,从exon 2到7的大部分区域被替换成一个带有floxed neo cassette的系统,同时加入了farnesyl化的EGFP。蛋白质表达缺失通过脑组织的 Western blot 分析得到了确认(来源:J:160509)
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The sequence spanning most of exon 2 through 7 was replaced with a floxed neo cassette and farnesylated EGFP. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:160509)