(转录起始区和外显子1被替换为一个含floxed neo标签的 cassette,随后通过Cre介导的重组移除了neo cassette。蛋白表达缺失通过脑组织的 Western blot 分析得到了确认。来源:J:160542)
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The promoter region and exon 1 were replaced with a floxed neo cassette. Cre mediated recombination removed the neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:160542)