2号和3号外显子通过同源重组被gfp-neo cassette替换了。蛋白缺失通过 Western blot 验证,且观察到了GFP表达(来源:J:161317)
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Exons 2 and 3 were replaced with a gfp-neo cassette by homologous recombination. Absence of protein was confirmed by western blot and GFP expression was observed. (J:161317)