通过Cre介导的重组,去除了neo cassette、exon 3和exon 4。蛋白表达缺失通过在胸腺和脾脏提取物中进行 Western blot 分析得到了确认(来源:J:159657)
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Cre mediated recombination removed the neo cassette, exon 3 and exon 4. The absence of protein expression was confirmed by western blot analysis on thymus and spleen extracts. (J:159657)