通过Cre介导的重组,去除了exon 4和neo cassette,蛋白表达缺失通过小肠、盲肠、结肠、胃和气管的提取物进行了 Western blot 验证(来源:J:160721)
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Cre mediated recombination removed exon 4 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on jejunum, ileum. colon, stomach, and trachea extracts. (J:160721)