由Cre介导的重组移除了exon 5。这个等位基因也缺失了neo cassette。蛋白质表达的缺失通过在小鼠胚胎成纤维细胞提取物中进行 Western blot 分析得到了确认。(来源:J:159889)
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Cre mediated recombination removed exon 5. This allele also lacks the neo cassette. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblast extracts. (J:159889)