The transgene contains the CMV enhancer/chicken beta-actin promoter, a floxed beta-geo reporter/SV40 polyadenylation signal segment, and murine Lhx2 cDNA, an internal ribosomal entry site (IRES) and green fluorescent protein (GFP) followed by a rabbit Hbb polyadenylation sequence. The cells with the highest lacZ expression and a single integration were selected for blastocyst injection. (J:159209)