开放阅读框被替换了为neo cassette。通过在生殖细胞中进行免疫组化和睾丸提取物的 Western blot 分析,证实了没有蛋白质表达。(来源:J:159793)
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The open reading frame was replaced with a neo cassette. The absence of protein expression was confirmed by immunohistochemistry on germ cells and western blot analysis on testes extracts. (J:159793)