Smcr8^{tm1(KOMP)Vlcg}

The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 2434 bp between positions 60668857-60671290 of Chromosome 11 (Build GRCm39). The gene locus is partially replaced by a cassette containing lacZ-polyA followed by a loxP-flanked hUbCpro-neo-polyA sequence. RT-PCR and Western blot analyses confirmed the absence of mRNA and protein expression in mouse embryonic fibroblasts (MEFs) derived from homozygous mutant embryos. (J:136110, J:236977)