这段基因序列,从exon 4到exon 7的大部分被lacZ和一个neo cassette替换了。通过脑和淋巴结细胞提取物的 Western blot 分析,证实了没有蛋白质表达(来源:J:159604)
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The genomic sequence spanning most of exon 4 through exon 7 was replaced with lacZ and a neo cassette. The absence of protein expression was confirmed by western blot analysis on brain and lymph node cell extracts. (J:159604)