通过Red/ET重组,构建了一个靶向载体,其中包含由Sykb调控元件驱动的Cre重组酶。载体中包含了FRT-flanked的Pgk-neomycin选择标记片段。这个载体通过电穿孔法导入R1 ES细胞。正确重组的克隆被注入C57BL/6的囊胚中以产生嵌合体。(来源:J:159112)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count