通过Red/ET重组,构建了一个靶向载体,其中包含由Sykb调控元件驱动的Cre重组酶。载体中包含了FRT-flanked的Pgk-neomycin选择标记片段。这个载体通过电穿孔法导入R1 ES细胞。正确重组的克隆被注入C57BL/6的囊胚中以产生嵌合体。(来源:J:159112)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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