通过Cre和Flp介导的重组,去除了修改的exon 14和neo cassette。通过脑组织提取物的 Western blot 分析,确实证实了截短蛋白表达的减少(来源:J:159311)
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Cre and flp mediated recombination removed the modified exon 14 and the neo cassette. Reduced expression of a truncated protein was confirmed by western blot analysis on brain extracts. (J:159311)