新霉素抗性基因连同3个串联的同义终止密码子插入到了第二个外显子中。 Western blot和免疫染色结果显示,纯合小鼠的肠道中没有蛋白表达。(来源:J:158733)
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The neomycin resistance gene along with 3 tandem in-frame stop codons was inserted into the second exon. Western blot and immunostaining indicated a lack of protein expression in the intestines of homozygous mice. (J:158733)