在翻译起始位点下游插入了带有SV40 3' UTR和一个floxed neo cassette的荧光素酶编码序列,它取代了前4个编码外显子。通过Cre介导的重组,neo cassette被去除了。蛋白表达的缺失通过胚胎纤维细胞提取物的Western blot分析得到了确认。(来源:J:158934)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count