Bid^tm1.1Ast

The allele was created by crossing heterozygous Bidtm1Ast mice with Tg(CMV-cre)1Cgn cre deleter mice to remove neo cassette, leaving a single loxP site and deleting approximately 4.5 kb region of the bid locus containing exons 1 and 2 plus part of exon 3, including the crucial BH3 domain. Western blot analysis on spleen and thymus samples and thymocytes derived from homozygous mice demonstrated that no detectable protein is produced from this allele. (J:158191)