一段exon 1被通过同源重组替换为GFP-Neo选择座。通过定量RT-PCR和 cerebellum提取物的 Western blot分析,确认了RNA和蛋白质的缺失。(来源:J:157850)
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A portion of exon 1 was replaced by a GFP-Neo selection cassette by homologous recombination. Absence of RNA and protein was confirmed by quantitative RT-PCR and western blot analysis of cerebellum extracts. (J:157850)