在目标基因编码C端区域的内含子右侧插入了loxP引导的诺卡因抗性基因座。通过PCR突变技术引入了人工终止密码子和额外的AatII限制酶位点,以去掉翻译出的蛋白质的最后7个氨基酸。在精原细胞中,通过Cre重组酶剪除诺卡因抗性基因座。(来源:J:183900)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
2
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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