Fgf8^tm2.1Jyhl

A point mutation into the 3' alternative splice acceptor of Fgf8 exon 1D, changing the sequence from cagCATGTG to caaCATGTG. A floxed neo selection cassette was place 400 bp downstream of exon 1D. The cassette was removed by cre mediated recombination. RT-PCR demonstrated that the transcripts of Fgf8a-containing spliceforms (a, c, e, and g) were missing, while Fgf8b-containing spliceforms, which utilize the remaining alternative splice acceptor of the exon 1D, are expressed. (J:156717)