整个编码序列(Exon 3)被替换了LacZ/Neo选择座。转录和蛋白的缺失通过RT-PCR和 Western blotting进行了验证。(来源:J:157383)
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The entire coding sequence, exon 3, was replaced by a LacZ/Neo selection cassette. Absence of transcript and protein were verified by RT-PCR and western blotting. (J:157383)