设计的一种靶向载体,从5'至3'包括一个splice接受器位点、一个由loxP标记的Neo-STOP插件、RACDA(RacG12V,通过甘氨酸替换缬氨酸使其在氨基酸12处失去活性,从而被转化为一种持续激活的Rac1突变体)的cDNA序列、一个frt标签的增强型绿色荧光蛋白(IRES-EGFP)插入,以及牛的poly(A)信号。这个转录本载体被插入Gt(ROSA)26Sor基因座的1号和2号外显子之间。(来源:J:157297)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
Targeted
插入
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7

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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